Altered extent of cross-linking of beta1,6-glucosylated mannoproteins to chitin in Saccharomyces cerevisiae mutants with reduced cell wall beta1,3-glucan content
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چکیده
منابع مشابه
Glycosyl phosphatidylinositol-dependent cross-linking of alpha- agglutinin and beta 1,6-glucan in the Saccharomyces cerevisiae cell wall
The cell adhesion protein alpha-agglutinin is bound to the outer surface of the Saccharomyces cerevisiae cell wall and mediates cell-cell contact in mating. alpha-Agglutinin is modified by addition of a glycosyl phosphatidylinositol (GPI) anchor as it traverses the secretory pathway. The presence of a GPI anchor is essential for cross-linking into the wall, but the fatty acid and inositol compo...
متن کاملRsc14-controlled expression of MNN6, MNN4 and MNN1 regulates mannosylphosphorylation of Saccharomyces cerevisiae cell wall mannoproteins.
ybl006cDelta was selected using an Alcian-blue-based screen aimed to identify nonessential genes involved in the regulation of mannosylphosphorylation. When cells of this deletant were mixed with the cationic dye Alcian blue in a typical assay, they remained white, indicating a low number of mannosylphosphate groups on the cell surface. ybl006cDelta cells did not show any defect in growth rate ...
متن کاملIncreased mortality of Saccharomyces cerevisiae cell wall protein mutants.
The yeast cell wall contains an unusually high number of different mannoproteins. The physiological role of most of them is unknown and gene disruptions leading to depletion of different proteins do not affect major functions of the wall. In this work the phenotype of different single and multiple cell wall protein mutants was observed at the level of individual cells. It was found that the lac...
متن کاملIdentification of three mannoproteins in the cell wall of Saccharomyces cerevisiae.
Three glucanase-extractable cell wall proteins from Saccharomyces cerevisiae were purified, and their N-terminal amino acid sequences were determined. With this information, we were able to assign gene products to three known open reading frames (ORFs). The N-terminal sequence of a 55-kDa mannoprotein corresponded with the product of ORF YKL096w, which we named CWP1 (cell wall protein 1). A 80-...
متن کاملSignalling towards cell wall synthesis in budding yeast.
The budding yeast Saccharomyces cerevisiae has long proved to be a very useful model in cell biology. Its cell morphology is established and maintained at least in part by the cell wall, a rigid but dynamic structure that affords mechanical protection. Although fungal cell walls represent an unique phenomenon, recent progress in research has shown striking parallels between yeast and mammalian ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1997
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.179.20.6279-6284.1997